Attenuation of -Amyloid-Induced Oxidative Cell Death by Sulforaphane via Activation of NF-E2-Related Factor 2
Figure 4
Inhibitory effect of SUL on the A-induced intracellular accumulation of ROS and oxidative stress. (a) SH-SY5Y cells were treated with 15 M A for 6 h with or without SUL pretreatment for 30 min. Intracellular ROS levels were monitored by using DCF-DA fluorescence dye. (A) Vehicle-treated control; (B) A alone (15 M); (C) A (15 M) + SUL (5 M). ((b)-(c)) SH-SY5Y cells were exposed to 15 M A in the presence or absence of SUL (2 M and 5 M) for 24 h. Molecular markers for oxidative damages such as lipid peroxidation (b) and protein oxidation (c) were determined by western blot analysis and protein carbonyl assay as described in Section 2. (d) The protein expression of antioxidant enzymes for example GCS, NQO-1, and HO-1 was evaluated by western blotting using their specific antibodies. Actin levels were assessed to confirm the equal amount of protein loaded.
