The Cell Wall Sensors Mtl1, Wsc1, and Mid2 Are Required for Stress-Induced Nuclear to Cytoplasmic Translocation of Cyclin C and Programmed Cell Death in Yeast
Figure 3
Mtl1 and Mid2/Wsc1 membrane sensors are required for cyclin C destruction following 0.4 mM H2O2 stress. (a) Wild type (RSY10) and mtl1Δ (RSY1660) expressing myc-cyclin C (pLR337) were grown to midlog phase (0 h) and then treated with 0.4 mM H2O2 for the indicated times in hours. Cyclin C levels were determined by Western blot analysis of immunoprecipitates. Tub1 levels were used as a loading control. (b) Cyclin C levels were monitored in mid2Δ (RSY1538), mid2Δ wsc1Δ (RSY1547), and mid2Δ wsc1Δ mtl1Δ (RSY1707) strains as described in (a). (c) Quantification of the results obtained in (a) and (b). (d) T-loop phosphorylation (top panel) and immunoprecipitations (bottom panel) of Slt2-HA in wild type, mtl1Δ, mid2Δ wsc1Δ, and (e) mid2Δ wsc1Δ mtl1Δ following 0.4 mM H2O2 treatment for the times indicated (h).
