Oxidative Medicine and Cellular Longevity / 2013 / Article / Tab 2 / Research Article
The Cell Wall Sensors Mtl1, Wsc1, and Mid2 Are Required for Stress-Induced Nuclear to Cytoplasmic Translocation of Cyclin C and Programmed Cell Death in Yeast Table 2 Primers used in this study.
Name Mutation created Oligonucleotide MID2 F1 mid2 Δ5′ TCGTTGAAGATTGGACATATAAAATACGCAAATCATAGTCGGATCCCCGGGTTAATTAA 3′ MID2 R1 mid2 Δ5′ AGGAATGAAAAGTAGCCATAAGCACTAAATGATATCAATGAATTCGAGCTCGTTTAAAC 3′ WSC1 F wsc1 Δ5′ GTA AAC TCG ACC AGG CAC 3′ WSC1 R wsc1 Δ5′ TATATCGTCTTTCAACGCGG 3′ MTL1 F1 mtl1 Δ5′ TTAACTTACTCCCAGTTAGTATAATATAAGTAGTTAAGGTCGGATCCCCGGGTTAATTAA 3′ MTL1 R1 mtl1 Δ5′ TTAAGAAGAAAAGTTATGGCAAAGCTGCTTTCGCTATGATGAATTCGAGCTCGTTTAAAC 3′ MTL1 F2 MTL1-3HA 5′ TATTACACGAAACCAAACAACGGCTTAAATATCACGAACTATCGGATCCCCGGGTTAATTAA 3′ MTL1 F4 PGAL-MTL1 5′ TTTAAACACTTCTAGTTCATTTCGGGTTGGTTCGATCTTGGAATTCGAGCTCGTTTAAAC 3′ MTL1 R2 PGAL-MTL1 5′ TTGAAGCAGAGCTCTTCTTCCTGGTCGGATTGCAGCTTGCCATTTTGAGATCCGGGTTTT 3′ N42A-F Mtl1N42A 5′ GCAGGAGTTGGTTCCAGCTGCTAGCACAACATCGAGCAC 3′ N42A-R Mtl1N42A 5′ GTGCTCGATGTTGTGCTAGCAGCTGGAACCAACTCCTGC 3′ N547A-F Mtl1N547A 5′ GAAACCAAACAACGGCTTAGCTATCACGAACTATTAAATC 3′ N547A-R Mtl1N547A 5′ GATTTAATAGTTCGTGATAGCTAAGCCGTTGTTTGGTTTC 3′ KCO1234 Amplify gfa1-1 5′ CGCTTACAAGAAAGCATTC 3′ KCO1235 Amplify gfa1-1 5′ GTCTAATTTAGGGCTGCAAC 3′
Oligonucleotides used in this study with their accompanying mutation identified.