Oxidative Medicine and Cellular Longevity / 2013 / Article / Fig 2

Research Article

Tanshinol Attenuates the Deleterious Effects of Oxidative Stress on Osteoblastic Differentiation via Wnt/FoxO3a Signaling

Figure 2

Protective effects of Tanshinol against H2O2-induced cell death and ROS generation. (a) Cell viability was measured by MTT assay in C2C12 cells exposed to H2O2 at the indicated concentration for 24 h. (b) C2C12 cells were pretreated in the presence or absence of Tanshinol (1 μM) for 1 h before the addition of H2O2 (200 μM) and were subsequently cultured for 0 h, 12 h, 24 h, 36 h, and 48 h, respectively. (c) The chemical structure of Tanshinol consists of polyphenolic hydroxyl groups. (d) C2C12 cells were pretreated as described in (b), followed by H2O2 (200 μM) for 24 h, and the oxidative status of C2C12 cells was quantified by DCHF-DA to monitor the emitted fluorescence intensity resulting from intracellular oxidation using flow cytometry. Note: (1) Con (vehicle control); (2) H (H2O2); (3) H + T (H2O2 + Tanshinol); (4) H + R (H2O2 + Resveratrol). Data are given as mean ± SEM of at least three independent experiments. versus vehicle control and versus H2O2 treatment.

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