Tanshinol Attenuates the Deleterious Effects of Oxidative Stress on Osteoblastic Differentiation via Wnt/FoxO3a Signaling
Tanshinol diminishes the cell cycle arrest and apoptosis under oxidative stress. C2C12 cells were pretreated with the indicated concentrations of Tanshinol or Resveratrol for 1 h, followed by the treatment of vehicle control or H2O2 for 24 h. The following measurements were carried out. (a) Analysis of cell cycle distribution was explored using flow cytometry; (b) the activation of caspase 3 was determined by ELISA kit; (c) observations of morphological alterations of apoptosis cells were addressed by Hoechst33258 staining (left panel) and counted by Image J software (right panel); (d) the ultrastructural differences between apoptosis cells and normal cells were examined using TEM. Note: (1) Con (vehicle control); (2) H (H2O2); (3) H + T (H2O2 + Tanshinol); (4) H + R (H2O2 + Resveratrol). Error bars indicate mean ± SEM of at least three independent experiments. versus vehicle control and versus H2O2 treatment. Original magnification ×400 in (c) (left panel).
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