Oxidative Medicine and Cellular Longevity / 2013 / Article / Fig 8

Research Article

Tanshinol Attenuates the Deleterious Effects of Oxidative Stress on Osteoblastic Differentiation via Wnt/FoxO3a Signaling

Figure 8

Effects of Tanshinol on targets genes of Wnt/Tcf and FoxO3a in C2C12 cells and MC3T3-E1 cells treated with FoxO3a siRNA, or with Tcf4 overexpression under oxidative stress. ((a), (c), (e), and (g)) C2C12 cells or MC3T3-E1 cells were transfected transiently with either the FoxO3a siRNA or the scrambled RNA control in the presence or absence of vehicle, H2O2, or Tashinol for 24 hours. The relative mRNA expression levels of Gadd45a, CAT, Axin2, and ALP genes in control cells (Scrambled) and in the FoxO3a-knockdown (FoxO3a siRNA) cells were assessed by quantitative RT-PCR assay. ((b), (d), (f), and (h)) C2C12 cells or MC3T3-E1 cells were transfected transiently with either the overexpression plasmid of Tcf4 or the empty vector control. The following procedure was executed as described in (a), (c), (e), and (g). mRNA values were normalized to GAPDH mRNA. Bars represent mean ± SME of three independent experiments. versus vehicle control and versus H2O2 treatment; versus corresponding empty vector or scrambled control.
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