PDGF Suppresses Oxidative Stress Induced Ca²⁺ Overload and Calpain Activation in Neurons

Figure 2

Effects of PDGF-BB on H₂O₂-induced calpain activation. Calpain-1 activity was measured 24 h after exposure to H₂O₂ as described in the Experimental procedures. (a) Calpain activity induced by the indicated amounts of H₂O₂ (5, 10, 20, 40, and 80 μM) exposure increased calpain activation in cortical neurons in a dose-dependent manner from 5–20 μM. ((b), (c)) Calpain activity in neuronal cultures pretreated with 50 ng/mL PDGF-BB 24 h (b) and 48 h (c) before exposure to H₂O₂ PDGF-BB completely blocked calpain activity induced by H₂O₂. Data are expressed as means ± SEM of three independent experiments. ^a1𝑃<0.01 and ^a2𝑃<0.05 versus the untreated control; ^b1𝑃<0.01 and ^b2𝑃<0.05 versus the same H₂O₂ exposure without the PDGF-BB pretreatment.

(a)

(b)

(c)