Differential MicroRNA Profiling in a Cellular Hypoxia Reoxygenation Model upon Posthypoxic Propofol Treatment Reveals Alterations in Autophagy Signaling Network
Figure 3
Propofol suppresses the H/R-induced autophagy. (a) The expression of LC3 was determined in normal cells, H/R injury cells, and propofol posthypoxia treatment cells (25–150 μmol/L). (b) The expression of LC3 was determined in control cells and cells treated by normoxia with propofol (25–150 μmol/L). (c) The expression of LC3 was determined in normal cells group, H/R injury cells group, and DMSO posthypoxia treatment groups (25–150 μmol/L). Data are representative WB from 3 independent experiments (). (d) Autophagosomes and mitochondria were probed by anti-LC3 and anti-Tim23 in normal cells, H/R injury cells, and propofol posthypoxia treated cells. Bar, 20 μm.
