Oxidative Medicine and Cellular Longevity / 2013 / Article / Fig 5

Research Article

Anti-Inflammatory Effect of Procyanidins from Wild Grape (Vitis amurensis) Seeds in LPS-Induced RAW 264.7 Cells

Figure 5

Effects of WGP on LPS-induced TNF-α and IL-1β protein expression in RAW 264.7 cells. The cells were treated with WGP (35 μg/mL) for 1 h and then treated with LPS for 24 h. Equal amounts of total protein were subjected to Western blot analysis, as described in Section 2. The ratio of immunointensity between the TNF-α/IL-1β and the β-actin was calculated. Each bar (open bar, TNF-α; closed bar, IL-1β) represents means ± SD from three independent experiments. indicates differences from the unstimulated control group. indicates differences from the LPS-treated group.
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