Curcumin Protects Human Keratinocytes against Inorganic Arsenite-Induced Acute Cytotoxicity through an NRF2-Dependent Mechanism
Figure 2
Effects of curcumin on protein expression of NRF2 and NRF1 in HaCaT cells (a) and (b). Representative images of western blot. HaCaT cells were exposed to indicate concentrations of curcumin for 6 hr (a) or 5 μM curcumin for the indicated period of time (b). Whole-cell lysates (50 μg protein) were separated on 8% Tris-Glycine gels and detected using anti-NRF2 or anti-NRF1. β-Actin was used as a loading control. Vehicle, medium. (c) Immunofluorescence staining of NRF2. HaCaT cells were treated with vehicle (left) or 5 μM curcumin (right) for 6 hr.
