Sulforaphane Enhances the Ability of Human Retinal Pigment Epithelial Cell against Oxidative Stress, and Its Effect on Gene Expression Profile Evaluated by Microarray Analysis
Figure 1
Sulforaphane (SF) endows the ability of antioxidative stress to RPE 19 cells from H2O2 induced apoptosis. Flow cytometry images and histogram presentations show cultured RPE 19 cells treated with or without Sulforaphane (SF) and H2O2. (a) Controls treated with PBS, or (b) SF only; (c) cells pretreated with PBS or (d) SF for 12 hours and then incubated with 400 μM H2O2 for 12 hours. Quadrant E1 (in cytometry images (a), (b), (c), and (d)) indicates cells undergoing apoptosis; E2 represents cells that died of secondary necrosis; E3 represents live cells; and E4 represents necrotic cells. The bar graph (e) shows RPE 19 cell apoptotsis under four different treatment conditions at four time points. The data came from four biological replicates, and mean ± SD of apoptotic rate are presented. Significant difference is indicated by asterisks; ** represents .
