Enhanced 4-Hydroxynonenal Resistance in KEAP1 Silenced Human Colon Cancer Cells

Figure 5

Induction of AKRs by H₂O₂ treatment. The sc and shKEAP1 HT29 cells were incubated with H₂O₂ (40 or 80 μM) for 24 h. The mRNA levels for AKR1C1 (a), 1C2 (b), 1C3 (c), 1B1 (d), and 1B10 (e) in the sc and shKEAP1 HT29 cells were assessed by using real-time PCR analysis. At the same incubation conditions, the protein levels for AKR1C1 and AKR1C2 were estimated following H₂O₂ (40 or 80 μM) incubation in the sc or shKEAP1 HT29 cells (f). The values are relative levels with respect to sc vehicle group and are the means ± SD of 3-4 experiments. ^a𝑃<0.05 compared with the sc group.

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