Dietary Flavonoids as Therapeutics for Preterm Birth: Luteolin and Kaempferol Suppress Inflammation in Human Gestational Tissues In Vitro
Figure 1
Effect of luteolin and kaempferol on proinflammatory cytokines. ((a), (b)) Fetal membranes were incubated with 10 μg/mL LPS in the absence or presence of 20 μM luteolin or 100 μM kaempferol for 24 h ( patients). (a) TNF-α, IL-6, and IL-8 mRNA expression were quantified by qRT-PCR. (b) IL-6 and IL-8 concentrations in the conditioned media were assayed using ELISA. Each bar represents the mean ± SEM. versus basal (one-way ANOVA). ((c), (d)) Primary myometrial cells were incubated with 500 pg/mL IL-1β in the absence or presence of 20 μM luteolin or 100 μM kaempferol for 24 h ( patients). (c) IL-6 and IL-8 mRNA expressions were quantified by qRT-PCR. (d) IL-6 and IL-8 concentrations in the conditioned media were assayed using ELISA. Each bar represents the mean ± SEM. versus basal (one-way ANOVA).
