Dietary Flavonoids as Therapeutics for Preterm Birth: Luteolin and Kaempferol Suppress Inflammation in Human Gestational Tissues In Vitro
Figure 3
Effect of luteolin and kaempferol on MMP-9 expression and activity. ((a), (b)) Primary amnion cells were incubated with 1 ng/mL IL-1β in the absence or presence of 20 μM luteolin or 100 μM kaempferol for 24 h ( patients). ((c), (d)) Primary myometrial cells were incubated with 500 pg/mL IL-1β in the absence or presence of 20 μM luteolin or 100 μM kaempferol for 24 h ( patients). ((a), (c)) MMP-9 mRNA expression was quantified by qRT-PCR. ((b), (d)) The incubation medium was assayed for MMP-9 activity by gelatin zymography. Zymography from one patient is shown.
