Mitochondrial DNA Instability in Cells Lacking Aconitase Correlates with Iron Citrate Toxicity
Figure 2
mtDNA is maintained in rtg1Δ aco1, rtg2Δ aco1Δ, and rtg3Δ aco1Δ mutant cells. (a) Cells grown exponentially in YPD medium were stained with DAPI and then examined using fluorescence microscopy. WT (rho+), BY4741; WT (rho0), BY4741 rho0; aco1Δ, ZLY2630; rtg1Δ aco1Δ, ZLY2648; rtg2Δ aco1Δ, ZLY2631; rtg3Δ aco1Δ, ZLY2652. (b) Quantitative analysis of the percentage of rho0 cells in yeast strains based on DAPI-staining images. (c) mtDNA in rtgΔ aco1Δ double mutant cells is functional. Strains as described in (a) as well as rtg1Δ, rtg2Δ, and rtg3Δ single mutants were crossed to a rho0 tester strain (X rho0) of opposite mating type. Diploids were selected and grown on YPD (Dextrose) and YPEthanol (Ethanol) medium. Pictures of cells were taken after 3 days’ growth at 30°C.
