Oxidative Stress and Mitogen-Activated Protein Kinase Pathways Involved in Cadmium-Induced BRL 3A Cell Apoptosis
(a) Effect of Cd and NAC on ERK and p38 phosphorylation of BRL 3A cells. (b) Effect of Cd on the JNK phosphorylation and Bax and Bcl-2 protein expression levels of BRL 3A cells. (c) Quantitative analysis of the immunoreactive phosphorylated ERK and p38 proteins in treated BRL 3A cells. (d) Quantitative analysis of the immunoreactive phosphorylated JNK, Bax, and Bcl-2 proteins in Cd-treated BRL 3A cells. Each value is expressed as the phospho/total MAPK percentage of phosphorylation and the ratio of OD in Bax and Bcl-2 with respect to β-actin. Significant difference: *, **, compared with the control. #, ##, compared with the 20 μmol/L Cd group.