Protective Effects of Low-Frequency Magnetic Fields on Cardiomyocytes from Ischemia Reperfusion Injury via ROS and NO/ONOO⁻

Figure 1

Identification of primary cardiomyocytes (CMs) and optimal low-frequency pulse magnetic fields (LFMFs) conditions. (a) Primary CMs by light microscope (scale bar, 100 μm). (b) cTnI staining of primary CMs by immunofluorescence (red, cTnI; blue, DAPI; scale bar, 50 μm). (c), (d) Cell viability measurement by MTT assays under different LFMFs conditions. ^∗𝑃<0.05 versus control group; ^#𝑃<0.05 versus H/R group. (e) Representative images of immunostaining for apoptotic cells (green, TUNEL; blue, DAPI; scale bar, 100 μm). (f) Quantification of apoptotic cells by Image-Pro Plus software. ^∗𝑃<0.05 versus control group; ^#𝑃<0.05 versus H/R group.

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