Research Article

Quercetin Induces Mitochondrial Mediated Apoptosis and Protective Autophagy in Human Glioblastoma U373MG Cells

Figure 3

Quercetin induces autophagy in U373MG cells. The cells were treated with various concentrations of quercetin for 48 h. (a) Analysis of the formation of autophagosomes and autolysosomes by fluorescence microscopy and flow cytometry. Representative areas were photographed with 200X magnification. (b) Quantification of acridine orange-positive cells. (c) Western blotting using antibodies specific for LC3, Beclin-1, and actin. Results shown are representative of three independent experiments. Images were captured using a fluorescence microscope. Results shown are representative of at least three replicates. All data are the mean ± standard deviation of three independent experiments. a, significantly different from the control, .