Figure 1: p70 expression in the cytosolic compartment of vascular smooth muscle cells—time-course and oxidant treatment. Panel (a) shows that mouse vSMCs were treated with 3 μM BaP or vehicle (DMSO) for 0.5 or 3 hr. Duplicate cell samples were processed for reproducibility. Panel (b) shows vSMCs were treated with 50 μM H2O2 or vehicle (ddH2O) for 0.5 and 3 hr. p70 expression was normalized against p35, a cellular protein recognized specifically by the antibody but not involved in the cellular response to oxidative stress. Densitometric values denote the average of duplicate samples from 3 independent experiments.