Enhanced Prostacyclin Synthesis by Adenoviral Gene Transfer Reduced Glial Activation and Ameliorated Dopaminergic Dysfunction in Hemiparkinsonian Rats
Figure 1
Analysis of 14C-labelled eicosanoids generated in control or Ad-PGIS-transduced mixed glial cultures in response to [1-14C] AA and COX inhibitors. Measurement of eicosanoid biosynthesis in cultures was conducted at 3 days after Ad-PGIS transduction. NS398 is a COX-2 specific inhibitor, and indomethacin is an inhibitor for both COX-1 and COX-2. Inhibitor was added to cultures 30 min before and during 14C-AA pulse. 6-KP denotes 6-keto-PGF1α, the product of PGI2 hydrolysis. Peaks of first 5-minute fractions are nonspecific. Each prostanoid peak was verified by coelution with an authentic radiolabelled prostanoid.
