Enhanced Prostacyclin Synthesis by Adenoviral Gene Transfer Reduced Glial Activation and Ameliorated Dopaminergic Dysfunction in Hemiparkinsonian Rats

Table 1

Effect of Ad-PGIS gene transfer on the proliferative activity in mixed glial cells.

	Thymidine incorporation	[^14C] 6-keto-PGF1α
	(cpm/well)
Control	378±19	100 ± 4
Ad-PGK	372±24	103 ± 2
Ad-PGIS	277±16 *	750 ± 5**

Subconfluent rat glial cells in 24-well plates or 6 cm dishes were infected with Ad (5×10⁵ pfu/w) for 2 days. [³H]-thymidine (0.5 𝜇Ci) was added to culture (in 24-well plate) 10 hrs before cell harvest. Trichloroacetic-acid-(TCA-) insoluble fraction (radioactivity) was collected and counted. For glial cells grown in 6 cm dishes, cells were incubated with 10 𝜇M [1-¹⁴C] AA for 10 min. [1-¹⁴C] AA metabolites in the medium were then processed for HPLC analysis. Data are expressed as means ± SEM from 4 independent repeats. *𝑃<0.05; **𝑃<0.01 by one way ANOVA.