Atherogenesis May Involve the Prooxidant and Proinflammatory Effects of Ferryl Hemoglobin

Figure 1

FerrylHb triggers oxidative modification of LDL. Human plasma was incubated with heme, Hb, metHb, and ferrylHb for 1 hour at 37°C, followed by separation of LDL. Concentrations are indicated and expressed as μmol/L heme groups. Following separation samples were kept at 4°C. Conjugated dienes ((a) and (d)), LOOH ((b) and (e)), and TBARs ((c) and (f)) in LDL samples were measured every day for 15 days. Results are representative of 3 independent experiments. Heme contents of the same samples were measured on the day of separation (g) and 15 days later (h). Data represent mean ± S.D. of 3 independent experiments.