Inhibition of Nuclear Nox4 Activity by Plumbagin: Effect on Proliferative Capacity in Human Amniotic Stem Cells
Figure 2
Nox4 expression in subcellular compartment of AFSC. (a) Representative images of Western blot analysis of total lysates (TL), cytosol (cyto) and nuclear fractions (nuclei) of AFSC revealed with different Nox4 antibodies: Santa Cruz (SC), Abcam (Abc), and Novus (Nov). Tubulin is shown as marker of nuclei purification. (b) Western blot analysis of nuclear lysate (NL) and immunoprecipitation experiment of NL with Nox4 antibody from Novus revealed with anti-Matrin, anti-Nox4, anti-ERK1/2, and anti-p22phox. Presented data are representative of three independent experiments.
