Oxidative Medicine and Cellular Longevity / 2013 / Article / Fig 5

Research Article

Inhibition of Nuclear Nox4 Activity by Plumbagin: Effect on Proliferative Capacity in Human Amniotic Stem Cells

Figure 5

Effect of plumbagin on AFSC proliferation. (a) AFSC (between the 6th and at 8th passages) were incubated for 24 hours with increasing concentration of plumbagin (0–5 μM). Cell viability was determined with the MTT test, as described in the Material and Methods section. *** ; ** ; * significantly different from control cells. (b) Cytofluorimetric analysis with propidium iodide (PI) of AFSC in culture from passages 8 to 12 in the presence or absence of 2 μM plumbagin. The graph shows the percentage of cells on S phase. Presented data are representative of three independent experiments. * significantly different from control cells. (c) Representative images showing superimposing between DAPI (blue) and Ki67 (green) signals of AFSC cultures at passages 8 and 12 in the presence or absence of 2 μM plumbagin for 24 hours. Scale bar: 10 μm.

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