Increased Nitroxidative Stress Promotes Mitochondrial Dysfunction in Alcoholic and Nonalcoholic Fatty Liver Disease
Figure 4
Translational research application of a redox proteomics approach by analyzing the oxidatively modified proteins in control and alcohol-exposed rats in the absence or presence of PUFA. Oxidatively modified mitochondrial proteins from each group were identified with a redox proteomics method using biotin-NM as a probe [106, 107], purified with streptavidin-agarose beads, then displayed on 2D gel, and stained with silver. The images of all gels were adjusted by optimizing the similar density of an endogenous, internal protein (*) in each gel. Protein spots in encircled areas in indicated samples reflect the appearance or disappearance of oxidized proteins depending on the treatment in each group, as described in and adapted from [137].
