Astragalus Polysaccharide Suppresses Skeletal Muscle Myostatin Expression in Diabetes: Involvement of ROS-ERK and NF-κB Pathways
Figure 5
Effects of APS on the MAPK expression in palmitate-stimulated C2C12 cells. C2C12 cells were incubated for 24 h in DMEM containing 5% BSA in either the presence (FFA-treated cells) or absence (control cells) of 0.5 mmol/L FFAs. Cells treated with APS were incubated with additional APS at the final concentration of 200 μg/mL. The expression of ERK, p38, and JNK and phosphorylated ERK, p38, and JNK were determined by western blot. Data are expressed as means ± SD of 6 different experiments. ### versus vehicle, *** versus palmitate. ERK: extracellular signal-regulated kinase; JNK: c-Jun amino-terminal kinases.
