Resveratrol Suppresses PAI-1 Gene Expression in a Human In Vitro Model of Inflamed Adipose Tissue
Figure 1
Resveratrol-dependent downregulation of PAI-1 mRNA and protein levels in SGBS adipocytes. SGBS adipocytes were incubated in adipogenic media with vehicle control (DMSO) or 100 μM resveratrol (Res) for the indicated time points. (a) PAI-1 mRNA levels were measured by semiquantitative RT-PCR. Sp1 was used as a reference gene. (b) A representative RT-PCR of PAI-1 and Sp1 mRNA levels after treatment with DMSO or 100 μM Res. (c) The accumulation of PAI-1 in the media was measured by ELISA after treatment with increasing doses of Res for 24 or 48 h.
