Oxidative Medicine and Cellular Longevity / 2013 / Article / Fig 3

Research Article

Resveratrol Suppresses PAI-1 Gene Expression in a Human In Vitro Model of Inflamed Adipose Tissue

Figure 3

Resveratrol abolished the MacCM-dependent PAI-1 induction in SGBS adipocytes. SGBS adipocytes were treated with the indicated doses of resveratrol (Res), 10% MacCM, or a combination of Res and 10% MacCM for 48 h. (a) PAI-1 mRNA levels were analyzed by qPCR and results were normalized to HPRT. *Significant difference untreated versus Res or MacCM, **significant difference MacCM treated versus MacCM + Res. (b) Total cell protein lysates were isolated and subjected to Western blot analysis using an antibody against PAI-1 and β-actin as a loading control. (c) Accumulation of PAI-1 protein in media was measured by ELISA. *Significant difference untreated versus Res or MacCM, **Significant difference MacCM treated versus MacCM + Res.
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