Oxidative Medicine and Cellular Longevity / 2013 / Article / Fig 4

Research Article

Resveratrol Suppresses PAI-1 Gene Expression in a Human In Vitro Model of Inflamed Adipose Tissue

Figure 4

The effects of resveratrol on PAI-1 gene expression in SGBS adipocytes are not mediated via SIRT1, AMPK and PI3K. (a) Where indicated SGBS adipocytes were treated with 10 μM sirtinol, resveratrol (Res, 100 μM), and MacCM (10%) for 48 h. (a) PAI-1 mRNA levels were analyzed with qPCR and results were normalized to HPRT. *significant difference untreated versus Res, sirtinol, or MacCM; **significant difference untreated versus Res + sirtinol or Res + MacCM; ***significant difference MacCM versus MacCM + Res or MacCM + sirtinol; #significant difference MacCM versus MacCM + Res + sirtinol. (b) SIRT1+/+ and SIRT1−/− mouse embryonic fibroblasts were treated with 100 μM resveratrol (Res) or vehicle control (DMSO) for 24 h. The PAI-1 and SIRT1 protein levels were measured by Western blot. *Significant difference untreated versus Res, **significant difference wild-type versus knockout cells. (c) Representative Western blot. (d) AMPKα1/2+/+ and AMPKα1/2−/− mouse embryonic fibroblasts were treated with 100 μM resveratrol (Res) or vehicle control (DMSO) for 24 h. The PAI-1 and AMPKα1/2 protein levels were measured by Western blot. *significant difference untreated versus Res, **significant difference wild type versus knockout cells. (e) Representative Western blot. (f) Where indicated SGBS adipocytes were treated with 20 μM LY294002, 100 μM resveratrol (Res), and 10% MacCM for 48 h. The PAI-1 mRNA levels were measured by qPCR and results were normalized to HPRT. *Significant difference untreated versus Res, LY294002, or MacCM; **significant difference untreated versus Res + LY294002 or Res + MacCM; ***significant difference MacCM versus MacCM + Res or MacCM + LY204002; #significant difference MacCM versus MacCM + Res + LY294002.
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