Oxidative Medicine and Cellular Longevity / 2013 / Article / Fig 5

Research Article

Resveratrol Suppresses PAI-1 Gene Expression in a Human In Vitro Model of Inflamed Adipose Tissue

Figure 5

Macrophage-conditioned media do not induce ROS formation in human adipocytes and the antioxidant transcription factor Nrf2 does not contribute to the resveratrol effects on PAI-1 gene expression. (a), (b) SGBS adipocytes were labelled with 2.5 μM CM-H2DCFDA and then treated with 50 μM H2O2 and 10% MacCM for 15 min. ROS production was analyzed by flow cytometry. (a) ROS-positive adipocytes after treatment with H2O2 and MacCM; *significant difference untreated versus H2O2. (b) Histograms of ROS-positive cell percentage in cells cultured in medium or treated with H2O2 for 15 min. (c) Nrf2+/+ and Nrf2 knock-down mouse embryonic fibroblasts were treated with 100 μM resveratrol (Res) or corresponding vehicle control (DMSO) for 24 h. The PAI-1 and Nrf2 protein levels were measured by Western blot. *Significant difference untreated versus Res, **significant difference wild type versus knockout cells. (d) Representative Western blot.
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