Oxidative Medicine and Cellular Longevity / 2013 / Article / Fig 6

Research Article

Resveratrol Suppresses PAI-1 Gene Expression in a Human In Vitro Model of Inflamed Adipose Tissue

Figure 6

Resveratrol-mediated suppression of NFκB DNA binding activity did not abrogate PAI-1 gene expression. (a) Electrophoretic mobility shift assay using a 5′-end-labelled consensus oligonucleotide for NFκB binding and nuclear extracts from SGBS adipocytes. SGBS adipocytes were treated with 10% MacCM, 100 μM resveratrol, 100 μM SC-514, or combination of them and then incubated for 1 h. The DNA-protein complexes were separated by electrophoresis on 5% native polyacrilamide gels and visualized by phosphoimaging. (b) SGBS adipocytes were treated with 10% MacCM, 100 μM resveratrol (Res), 100 μM SC-514, or a combination of both Res and 10% MacCM or SC-514 and MacCM for 48 h. Accumulation of PAI-1 protein in media was measured by ELISA. *Significant difference untreated versus MacCM, Res or SC-514; **significant difference MacCM treated versus MacCM + Res or MacCM + SC-514.

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