Oxidative Medicine and Cellular Longevity / 2013 / Article / Fig 1

Research Article

The NRF2 Activation and Antioxidative Response Are Not Impaired Overall during Hyperoxia-Induced Lung Epithelial Cell Death

Figure 1

Hyperoxia induced cell death in AECs. Cells were exposed to hyperoxia (hyp) or room air (RA) for up to 48 h, and cell viability was measured at the indicated time points. Cell viability assay was evaluated with CellTiter Glo Reagent (a), MTT reagent (b), and LDH release into the culture medium (c). Results are represented as the percent viability of cells (a and b) or LDH release (c) in comparison to their time matched RA control groups. The graph represents mean ± SD of 4 independent experiments performed in triplicate. values are calculated using two-way ANOVA by Prism software. , RA versus hyp; §: , 24 h hyp versus 36 h hyp or 36 h hyp versus 48 h hyp.

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