Oxidative Medicine and Cellular Longevity / 2013 / Article / Fig 3

Research Article

The NRF2 Activation and Antioxidative Response Are Not Impaired Overall during Hyperoxia-Induced Lung Epithelial Cell Death

Figure 3

The effects of chronic hyperoxia on NRF2 target gene expression in AECs. Cells were exposed to either room air or hyperoxia for 36 or 48 h and then harvested for total RNA and protein. (a) HMOX1, GCLC, and NQO mRNA expression. The values represent the mean ± SD of four independent experiments ( ). , RA versus hyperoxia. (b) Western blot analysis of whole-cell lysates using anti-HMOX1, anti-NQO1, or anti-GCLC antibodies. Membranes were stripped and reprobed with -actin. Graph represents the relative band intensities of HMOX1, NQO, or GCLC, as quantified in Figure 2. Values represent the mean ± SD of four independent experiments ( ). , RA versus hyperoxia.
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