Oxidative Medicine and Cellular Longevity / 2013 / Article / Fig 4

Research Article

The NRF2 Activation and Antioxidative Response Are Not Impaired Overall during Hyperoxia-Induced Lung Epithelial Cell Death

Figure 4

The effects of acute and chronic hyperoxia on nuclear NRF2 levels. Cells were exposed to room air (RA) or hyperoxia for indicated time points and nuclear extracts were prepared. Equal amount of protein (~15 μg) was separated on SDS-PAGE and probed with anti-NRF2 antibody. Membrane was stripped and re-probed with anti-lamin B antibody, as loading control. (a). Nuclear NRF2 levels in cells exposed to hyperoxia for 1 to 12 h. (b) Nuclear NRF2 levels in cells exposed to hyperoxia for 36 h or 48 h. Graph represents the relative NRF2 band intensity of three independent experiments ± SD ( ). The band intensities of NRF2 were quantified using Image J software and normalized with their respective lamin B band intensities. NRF2 band intensities of room air-exposed samples considered as one arbitrary unit.

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