Curcumin Pretreatment Induces Nrf2 and an Antioxidant Response and Prevents Hemin-Induced Toxicity in Primary Cultures of Cerebellar Granule Neurons of Rats
Figure 2
Effect of curcumin on cerebellar granule neurons (CGNs) viability in absence or presence of hemin. (a) Bright field representative images (40x) of CGNs treated with curcumin (0–50 μM for 24 h). Scale bar represents 10 μm and applies to all panels. (b) Viability was quantified by fluorescein diacetate (FDA) fluorescence (∘) and 3-[4,5-dimethylthiazol-|2-yl)]-2,5-diphenyl-tetrazolium bromide (MTT) reduction (•). (c) CGNs were incubated with 5, 10, and 15 μM curcumin for 24 h before the addition of 30 μM hemin for 1 h. Subsequently hemin was replaced by fresh medium and the incubation was continued up to 24 h. Finally, cell viability was quantified by MTT reduction and expressed as percentage of control. Data are expressed as mean ± SEM, . versus 0 μM, versus control (untreated), versus hemin.
