Curcumin Pretreatment Induces Nrf2 and an Antioxidant Response and Prevents Hemin-Induced Toxicity in Primary Cultures of Cerebellar Granule Neurons of Rats

Figure 3

Effect of curcumin (C) pretreatment on hemin (H) induced reactive oxygen species (ROS) production in CGNs. (a) Representative images (40x) after 24 h of treatment with 5, 10, and 15 μM curcumin and exposed to 30 μM hemin for 1 h. Bright-field (left panel) and ethidium fluorescence (right panel). The same field is shown in each condition. Scale bar represents 10 μm and applies to all panels. (b) Intensity of ethidium fluorescence was measured in 5 different fields per well per condition. Data are expressed as mean ± SEM, 𝑛=3. ^∗𝑃<0.05 versus control, ^∗∗𝑃<0.05 versus hemin.

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