Curcumin Pretreatment Induces Nrf2 and an Antioxidant Response and Prevents Hemin-Induced Toxicity in Primary Cultures of Cerebellar Granule Neurons of Rats
Figure 4
Curcumin increases heme oxygenase-1 (HO-1) protein and glutathione (GSH) and glutathione disulfide (GSSG) levels in CGNs. (a) Curcumin induced HO-1 expression in a concentration (after 24 h incubation) and (b) time-dependent (with 15 μM curcumin) manner. Upper panels show graphs of densitometric analysis (HO-1/tubulin) from each band; lower panels show immunoblot of HO-1 and loading control with tubulin. (c) GSH and GSSG levels were determined 24 h after treatment with 5–30 μM curcumin. (d) [GSH] + [GSSG] content was evaluated 24 h after incubation with curcumin. Data are expressed as mean ± SEM, = 4-5. versus 0 μM curcumin or 0 hours.
