Curcumin Pretreatment Induces Nrf2 and an Antioxidant Response and Prevents Hemin-Induced Toxicity in Primary Cultures of Cerebellar Granule Neurons of Rats

Figure 5

Curcumin prevents hemin-induced changes in glutathione (GSH) and glutathione disulfide (GSSG) levels in CGNs. Cells were incubated with 15 μM curcumin for 24 h. The culture medium was removed and 30 μM hemin was added for 1 h. Hemin was replaced by fresh medium and incubation was continued up to 24 h. (a) GSH levels were assayed with monochlorobimane. (b) GSSG levels were quantified by using 2-vinylpriridine (2-VP). (c) [GSH]/[GSSG] ratio was calculated. (d) [GSH] + [GSSG] content was quantified by 5,5′-dithio-bis(2-nitrobenzoic acid) (DTNB). Buthionine sulfoximine (BSO), an inhibitor of GSH synthesis, was used as a control. Data are expressed as mean ± SEM, 𝑛=5. ^∗𝑃<0.05 versus control (untreated), ^#𝑃<0.05 versus hemin.

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