Curcumin Pretreatment Induces Nrf2 and an Antioxidant Response and Prevents Hemin-Induced Toxicity in Primary Cultures of Cerebellar Granule Neurons of Rats

Figure 8

Curcumin increased activity of antioxidant enzymes in CGNs. (a) Glutathione reductase (GR), (b) glutathione-S-transferase (GST) and (c) superoxide dismutase (SOD). CGNs were treated with 15 μM curcumin for 24 h. The culture medium was removed and then 30 μM hemin was added for 1 h. Hemin was subsequently replaced by fresh medium and the incubation was continued. Recovery time 24 h after exposition to hemin or curcumin was considered (see legend to Figure 7). Data are expressed as mean ± SEM, 𝑛 = 3–5. ^∗𝑃<0.05 versus control (untreated).

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