Ethanol and Acetate Acting as Carbon/Energy Sources Negatively Affect Yeast Chronological Aging

Table 1

pH values of exhausted media.

Days	wt	fps1Δ	icl1Δ	wt + pyrazole	icl1Δ + pyrazole
0	3.21±0.07	3.20±0.06	3.11±0.06
1	3.18±0.04	3.16±0.03	3.08±0.05	3.18±0.05	3.08±0.07
2	3.13±0.06	3.08±0.04	2.98±0.05	3.11±0.04	2.89±0.04
3	2.97±0.05	2.90±0.04	2.86±0.06	2.93±0.06	2.75±0.07
4	2.81±0.03	2.68±0.06	2.68±0.04	2.76±0.07	2.63±0.06
5	2.72±0.06	2.56±0.06	2.53±0.06	2.65±0.04	2.48±0.07
6	2.70±0.06	2.55±0.05	2.49±0.07	2.63±0.07	2.43±0.07

pH of the exhausted media was measured starting from diauxic shift, Day 0. Data presented are the mean values of three biological replicates. Standard deviations are indicated.