Mitogenesis of Vascular Smooth Muscle Cell Stimulated by Platelet-Derived Growth Factor-bb Is Inhibited by Blocking of Intracellular Signaling by Epigallocatechin-3-O-Gallate
Figure 1
The antiproliferative activity and cell cycle arrest activity by PDGF-bb on EGCG preincubated RAOSMC. After 24 h of starvation with DMEM containing increasing concentrations (10–80 μM) of EGCG, cells at 80% confluence were washed and treated with 10 ng/mL PDGF-bb. (a) The effects of growth inhibition on PDGF-bb stimulation in EGCG preincubated RAOSMC. Cell viability was detected using the MTT assay. * compared with nonstimulation control; # compared with the 10 ng/mL PDGF-bb stimulated control. (b) The effect of EGCG preincubation on PDGF-bb-induced DNA synthesis in RAOSMC. DNA synthesis was detected using the BrdU incorporation assay. * compared with nonstimulation control; # compared with 10 ng/mL PDGF-bb stimulated control. (c) EGCG preincubation with PDGF-bb stimulated cell cycle distribution in RAOSMC. Cell cycle distribution was determined by propidium iodide (PI) labeling followed by flow cytometry. The percentages of cells in the G0/G1, S, and G2/M phases were calculated using Modifit computer software and represented within the histograms.
