Transient Limb Ischemia Alters Serum Protein Expression in Healthy Volunteers: Complement C3 and Vitronectin May Be Involved in Organ Protection Induced by Remote Ischemic Preconditioning
Figure 3
Western blotting data of complement C3, vitronectin, and apoA-I. The results were presented as mean ± SD. Error bars were SD. Repeated-measures analysis of variances was performed to evaluate statistical significance.. (a) Representative western blotting images of serum samples from healthy volunteers obtained prior to transient limb ischemia (base) and at various time points (1 h, 3 h, 8 h, 24 h, and 48 h) thereafter. Memcode was shown to demonstrate equal protein loading. (b) Changes in the expression of complement C3 after transient limb ischemia compared with that before transient limb ischemia (). (c) Changes in the expression of vitronectin at 75 kDa after transient limb ischemia compared with that before transient limb ischemia (). (d) Changes in the expression of vitronectin at 65 kDa after transient limb ischemia compared with that before transient limb ischemia (). (e) Changes in the expression of apoA-I after transient limb ischemia compared with that before transient limb ischemia ().
