Effects of Resveratrol on Vitrified Porcine Oocytes
Figure 1
((a)–(c)) Fluorescent micrographs of oocytes after combined staining with Annexin V/Hoechst 33342 (Ho)/PI. (a) Live non apoptotic oocytes with Ho positive nuclei and no annexin staining (A−/PI−); (b) Live apoptotic oocytes with Ho positive nuclei and annexin positive signal on the membrane (A+/PI−); (c) Dead oocyte which showed PI positive red nuclei (PI+). ((d)–(f)) Representative fluorescent micrographs of pig oocytes after FITC-VAD-FMK, Ho and PI staining. (d) Live non apoptotic oocytes with Ho positive nuclei and no FITC-VAD-FMK staining (VAD−/PI−); (e) Live apoptotic oocyte with the cytoplasm stained in green by FITC-VAD-FMK (VAD+/PI−); (f) Dead oocyte with the metaphase plate stained in red by PI (PI+).
