Thiol Redox Sensitivity of Two Key Enzymes of Heme Biosynthesis and Pentose Phosphate Pathways: Uroporphyrinogen Decarboxylase and Transketolase

Figure 5

Alignment of uroporphyrinogen decarboxylase from S. cerevisiae and human. Hem12p is the enzyme from S. cerevisiae UniProt P32347 and UROD is from human, UniProt P06132. Cysteines are highlighted in yellow; dark grey shadowed residues indicate identical positions; tryptic Cys-peptides in Hem12p are highlighted in blue; thin red square marks Cys26 and its equivalent Cys35 in UROD; thick red square marks the conserved cysteine, Cys52 in Hem12p and Cys59 in UROD, respectively; green lines indicate the sequence signatures for Uroporphyrinogen decarboxylase UROD_1 and UROD_2 (PS00906 and PS00907, resp., according to ProSite). The sequences have been aligned using the program Clustal-ω and share 49.60% identity.