Thiol Redox Sensitivity of Two Key Enzymes of Heme Biosynthesis and Pentose Phosphate Pathways: Uroporphyrinogen Decarboxylase and Transketolase
Figure 6
Deglutathionylation of fluorescently labelled proteins (with eosin-GSH) by Grx2p system. Increase in fluorescence emission by free eosin-GSH is given in arbitrary units. BSA labelled with eosin-GSH and then incubated with complete Grx2p system or not are used as the positive (BSA+) and negative (BSA−) controls, respectively.