Research Article
Thiol Redox Sensitivity of Two Key Enzymes of Heme Biosynthesis and Pentose Phosphate Pathways: Uroporphyrinogen Decarboxylase and Transketolase
Table 1
Label-free MS quantification of reversibly oxidised Cys residues of Hem12p and Tkl1p using Progenesis. Peptides detected in a shotgun proteomic screen of reversibly oxidised Cys residues using the redox affinity enrichment approach in WT and ΔGrx2 yeast strains ±1 mM H2O2. Units are arbitrary for intensity of parent ions.
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*Hem12p had a sixfold increase in the detection of reversibly oxidised Cys26 in the ΔGrx2 strain compared to WT (). **Tkl1p was increased in both strains after H2O2 treatment and more significantly in the ΔGrx2 strain; this peptide was detected with () and (). Peak intensities are given in arbitrary units ×104 (Tkl1p) and ×103 (Hem12p). |