Research Article

Reversal of Myoblast Aging by Tocotrienol Rich Fraction Posttreatment

Figure 2

Effects of SIPS and TRF on myoblasts phenotype. The myoblasts were stained with an antibody against desmin (green) in the (a) young control, (b) SIPS-induced cells, (c) TRF control group, (d) pretreated cells with TRF, and (e) posttreated cells with TRF (magnification 400x). The nuclei were stained with Hoechst (blue). SIPS significantly induced the cells to become larger and flatter and the intermediate filament to become more prominent as compared to the young control. Pretreatment cells failed to keep their morphology in spindle shape as compared to the young control (d). On contrary, some of the posttreated cells with TRF remained spindle shaped which resembled young control while some exhibited flatter and larger morphology (e).
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