Activation of the Nrf2 Pathway by Inorganic Arsenic in Human Hepatocytes and the Role of Transcriptional Repressor Bach1
Figure 2
NaAsO2 induces Nrf2 mRNA expression in Chang human hepatocytes. Chang human hepatocytes were treated with NaAsO2 (10, 25, and 50 μmol/L) for 6 h. Total RNA was isolated and Nrf2 mRNA expression was conducted by real-time PCR. Primers for human Nrf2 gene was synthesized by Takara as described in Section 2. Triplicate reactions were performed for each sample. Data were from three different samples. Cycle threshold (Ct) values obtained graphically were used to represent the amounts of Nrf2 and GAPDH genes. Nrf2 mRNA levels were normalized to that of GAPDH and finally expressed as ratio to control cells. versus control cells.
