Activation of the Nrf2 Pathway by Inorganic Arsenic in Human Hepatocytes and the Role of Transcriptional Repressor Bach1
Figure 3
NaAsO2 increases ARE-luciferase activity with a dose-effect response in Chang human hepatocytes. The Cignal Lenti ARE reporter is a ready-to-transduce ARE-responsive lentiviral firefly luciferase reporter designed to monitor the transcriptional activity of Nrf2. Transfected Chang human hepatocytes were seeded in 96-well plates and then exposed to (a) tBHQ (10 and 50 μmol/L) for 6 h or (b) NaAsO2 (10, 25, and 50 μmol/L) for 6 h, respectively. Luciferase activity was measured using the Luciferase Reporter Gene Assay Kit, normalized to cell viability and finally expressed as ratio to control cells. Data were mean ± standard deviations (SD) from five different samples. versus control cells.
