Activation of the Nrf2 Pathway by Inorganic Arsenic in Human Hepatocytes and the Role of Transcriptional Repressor Bach1

Figure 4

NaAsO₂ induces Nrf2-regulated NQO1 and HO-1 expressions in Chang human hepatocytes. Chang human hepatocytes were treated with NaAsO₂ (10, 25, and 50 μmol/L) for 6 h. (a) The proteins from whole-cell extracts were used to detect the expression of NQO1 and HO-1 by western blot, with β-actin as an internal control. (b) and (c) were quantitative analysis of NQO1 and HO-1 proteins as shown in (a) and finally expressed as ratio to control cells. The mRNA levels of (d) NQO1 and (e) HO-1 genes were measured by real-time PCR, normalized to GAPDH mRNA levels, and finally expressed as ratio to control cells. Data were mean ± standard deviations (SD) from three different samples. ^∗𝑃<0.05 versus control cells.

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